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Meget følsom måling af glomerulær permeabilitet hos mus med fluorescein isothiocyanat-polysakkarose 70
JoVE Journal
Medicine
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JoVE Journal Medicine
Highly Sensitive Measurement of Glomerular Permeability in Mice with Fluorescein Isothiocyanate-polysucrose 70
DOI:

09:16 min

August 09, 2019

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Chapters

  • 00:04Title
  • 00:28Preparation Phase Urinary Catheter
  • 01:43Preparation Phase – Central Venous Catheter
  • 04:16Blood Pressure Measurement and Equilibration Phase
  • 05:51Experimental Phase
  • 07:10Results: Fluorescence Scan, Glomerular Permeability and Blood Pressure
  • 09:00Conclusion

Summary

Automatic Translation

Her præsenterer vi en protokol til at teste glomerulær permeabilitet i mus ved hjælp af en meget følsom, ikke-radioaktiv Tracer. Denne metode giver mulighed for gentagne urinanalyser med små urin volumener.

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