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小鼠模型中生物合成组织工程气管移植的育苗与植入
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Seeding and Implantation of a Biosynthetic Tissue-engineered Tracheal Graft in a Mouse Model
DOI:

09:57 min

April 01, 2019

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Chapters

  • 00:04Title
  • 01:15Scaffold Manufacturing
  • 02:50Bone Marrow-Derived Mononuclear Cell (BM-MNC) Harvest
  • 04:40Cell Seeding
  • 05:21Graft Implantation
  • 08:28Results: Representative Basal Epithelial Cell and Stenotic Graft Region Analyses
  • 09:27Conclusion

Summary

Automatic Translation

接枝狭窄是组织工程气道置换术的关键障碍。为了研究狭窄的细胞机制, 我们利用组织工程气管替代种子骨髓单个核细胞 (BM-MNC) 的小鼠模型。在这里, 我们详细介绍了我们的协议, 包括支架制造、BM-MNC 隔离、接枝播种和植入。

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