/
/
Såing og implantering av en biosyntetiske vev-konstruert Tracheal pode i en musemodell
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Otolaryngology Head & Neck Surgery,Nationwide Children’s Hospital, 2The Ohio State University College of Medicine, 3Center for Regenerative Medicine,Research Institute at Nationwide Children’s Hospital, 4Center for Perinatal Research,Nationwide Children’s Hospital, 5Nanofiber Solutions, Inc., 6Department of Pediatric Surgery,Nationwide Children’s Hospital
Chapters
- 00:04Title
- 01:15Scaffold Manufacturing
- 02:50Bone Marrow-Derived Mononuclear Cell (BM-MNC) Harvest
- 04:40Cell Seeding
- 05:21Graft Implantation
- 08:28Results: Representative Basal Epithelial Cell and Stenotic Graft Region Analyses
- 09:27Conclusion
Pode stenose utgjør en viktig hindring i vev utvikling airway erstatning. For å undersøke mobilnettet mekanismene bak stenose, benytter vi en murine modell av vev utvikling tracheal erstatning med seeded benmarg mononukleære celler (BM-MNC). Her, detalj vi våre protokollen, inkludert stillaset produksjon, BM-MNC isolasjon, pode såing og implantasjon.
