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Neuroscience
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小鼠耳蜗特定频率区域带状带突起的形态和功能评估
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Morphological and Functional Evaluation of Ribbon Synapses at Specific Frequency Regions of the Mouse Cochlea
Please note that all translations are automatically generated.
Click here for the English version.
小鼠耳蜗特定频率区域带状带突起的形态和功能评估
DOI:
10.3791/59189-v
•
09:54 min
•
May 10, 2019
•
Shu-Kui Yu*
1
,
Zheng-De Du*
1
,
Qing-Ling Song
,
Teng-Fei Qu
,
Yue Qi
,
Wei Xiong
,
Lu He
,
Wei Wei
,
Shu-Sheng Gong
,
Ke Liu
1
Department of Otolaryngology-Head and Neck Surgery, Beijing Friendship Hospital
,
Capital Medical University
,
2
Department of Otology
,
Shengjing Hospital of China Medical University
Chapters
00:04
Title
00:50
Animal Selection
02:12
Cochlear Tissue Processing
04:08
Immunofluorescence Staining
05:53
Morphological Cochlear Ribbon Synapse Evaluation
07:37
Results: Representative Cochlear Frequency Localization and Cochlear Ribbon Analysis
09:08
Conclusion
Summary
Automatic Translation
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Automatic Translation
本手稿描述了用于评估正常小鼠带状突触的形态特征和功能状态的实验协议。本模型也适用于噪声诱发和与年龄相关的耳蜗性突触性受限模型。讨论了以往小鼠研究的相关结果。
Tags
Cochlea
Frequency Region
Synapse
Neuropathy
Hearing Loss
Tinnitus
Hyperacusis
Auditory Brainstem Response
ABR
Cochlea Dissection
Paraformaldehyde
EDTA
Microscopy
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