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Isolating Malignant and Non-Malignant B Cells from lck

Isolating Malignant and Non-Malignant B Cells from lck:eGFP Zebrafish

Article doi: 10.3791/59191
February 22nd, 2019

Summary February 22nd, 2019

Transgenic lck:eGFP zebrafish express GFP highly in T lymphocytes, and have been used to study T cell development and acute lymphoblastic leukemia. This line can be used to study B cells, which express lck at lower levels. This protocol describes purification of malignant and non-malignant B cells from lck:eGFP zebrafish.


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