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Journal
/
Genetics
/
이류성 에 대한 착상 전 유전자 검사를위한 반도체 시퀀싱
JoVE Journal
Genetics
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JoVE Journal
Genetics
Semiconductor Sequencing for Preimplantation Genetic Testing for Aneuploidy
Please note that all translations are automatically generated.
Click here for the English version.
이류성 에 대한 착상 전 유전자 검사를위한 반도체 시퀀싱
DOI:
10.3791/59273-v
•
00:09 min
•
August 25, 2019
•
Baoheng Gui*
1,2,3
,
Yingxin Zhang*
4
,
Bo Liang
,
Yvonne Ka Yin Kwok
,
Wai Ting Lui
,
Queenie Sum Yee Yeung
,
Lingyin Kong
,
Liming Xuan
,
Jacqueline Pui Wah Chung
,
Kwong Wai Choy
4
1
Department of Genetics and Metabolism
,
Maternal and Child Health Hospital of Guangxi Zhuang Autonomous Region
,
2
Birth Defects Prevention and Control Institute of Guangxi Zhuang Autonomous Region
,
3
Shenzhen Research Institute
,
The Chinese University of Hong Kong
,
4
Department of Obstetrics and Gynaecology
,
The Chinese University of Hong Kong
,
5
State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology
,
Shanghai Jiao Tong University
,
6
Basecare Medical Device Co., Ltd
Chapters
00:04
Title
01:26
Library Pooling and Emulsion PCR Using an Emulsion System
03:21
Enrichment System (ES) Setup
04:29
Template Preparation
05:17
Sample Loading and Sequencing
07:56
Results: Next-generation Semiconductor Sequencing for PGT-A
08:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
여기서는 짧은 처리 시간, 낮은 비용 및 높은 처리량의 장점을 가진 이수성 (PGT-A)에 대한 착상 전 유전자 검사를위한 반도체 염기서열 분석 방법을 소개합니다.
Tags
Semiconductor Sequencing
Preimplantation Genetic Testing
Aneuploidy
Rapid
Low-cost
Template Amplification
Sequencing Library
PGTA Detection
High Throughput
Cost Efficient
Time Saving
Turnaround Time
Genome Sequencing
Copy Number Variation
Single Nucleotide Polymorphism
Sequencing Chemistry
Non-invasive Prenatal Screening
Library Preparation
Recovery Tubes
Recovery Router
Amplification Plate
Master Mix PCR Buffer
Sphere Particles
Ligation Mix
Reaction Filter
Proton Program
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