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使用免疫过氧化物酶和免疫荧光方法鉴定成年斑马鱼中的奥列辛和内他大麻素受体
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods
Please note that all translations are automatically generated. Click here for the English version.

使用免疫过氧化物酶和免疫荧光方法鉴定成年斑马鱼中的奥列辛和内他大麻素受体

DOI:
10.3791/59308-v

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08:37 min

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June 25, 2019

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, , , ,
1Department of Sciences and Technologies (DST),University of Sannio, 2Endocannabinoid Research Group,Institute of Biomolecular Chemistry, 3Department of Veterinary Medicine and Animal Productions,University of Naples Federico II

Chapters

  • 00:04Title
  • 01:13Tissue Cutting
  • 01:45Blocking of Nonspecific Binding Sites and Tissue Permeabilization
  • 02:44Incubation with Antibodies
  • 03:18Tissue Mounting
  • 03:56Controls
  • 04:26Image Acquisition
  • 05:31Results: Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish
  • 07:18Conclusion

Summary

Automatic Translation

Automatic Translation

这里介绍的是免疫组织化学表征和定位的orexin肽,orexin受体,和内分泌大麻素受体在肠道和大脑中正常和饮食诱发肥胖(DIO)成年斑马鱼模型使用免疫渗透酶和双免疫荧光法。

Tags

ImmunohistochemistryImmunoperoxidaseImmunofluorescenceZebrafishOrexinEndocannabinoid ReceptorsCryosectioningBlockingPrimary AntibodiesIncubation

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