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Bioengineering
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使用多光谱成像流细胞测定法执行体外微核测定的自动化方法
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
An Automated Method to Perform The In Vitro Micronucleus Assay using Multispectral Imaging Flow Cytometry
Please note that all translations are automatically generated.
Click here for the English version.
使用多光谱成像流细胞测定法执行体外微核测定的自动化方法
DOI:
10.3791/59324-v
•
12:56 min
•
May 13, 2019
•
Matthew A. Rodrigues
1
Biology Department
,
Luminex Corporation
Chapters
00:04
Title
00:33
Clastogen and/or Aneugen Exposure
01:49
Sample Processing
03:50
Multispectral Imaging Flow Cytometry (MIFC)
05:15
Binucleated Cell (BNC) Identification
06:48
Micronucleus (MN) Identification
07:53
Polynucleated Population Identification
08:41
BNC and MN Mask Examination
09:52
Statistics Table Creation
10:31
Experimental File Batch Processing
11:10
Results: Representative Cell Scoring and Cytotoxicity Quantification
12:23
Conclusion
Summary
Automatic Translation
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Automatic Translation
体外微核测定是评估基因毒性和细胞毒性的成熟方法,但使用人工显微镜对测定进行评分是费力的,具有主观性和分时变异性。本文介绍了为使用多光谱成像流细胞测定执行全自动测试而开发的协议。
Tags
In Vitro Micronucleus Assay
Multispectral Imaging Flow Cytometry
Automated Method
Genotoxicity
Cytotoxicity
Clastogens
Aneugens
Cell Exposure
Cytochalasin B
Potassium Chloride
Formalin
Hoechst 33342
High-throughput
Automated Microscopy
Conventional Flow Cytometry
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