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En Guide til å bygge et sterkt tilbøyelig feide flis mikroskop for utvidet felt-of-view Single-molekylet Imaging
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal Bioengineering
A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
DOI:

08:13 min

April 08, 2019

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Chapters

  • 00:04Title
  • 00:28Preliminary Setup
  • 01:11Setting up the Detection Path
  • 02:14Setting up the Excitation Path and the Cylindrical Lenses
  • 04:59Testing Tile Imaging
  • 05:53Highly Inclined Swept Tile (HIST) Imaging
  • 06:52Results: Highly Inclined Swept Tile Microscopy of Labeled DNA and RNA
  • 07:38Conclusion

Summary

Automatic Translation

En detaljert instruksjon er beskrevet på hvordan bygge et sterkt tilbøyelig feide flis (HIST) mikroskop og bruksområdene for single-molekylet bildebehandling.

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