Journal
/
/
单分子荧光原位杂交 (SM-FISH) 在小鼠卵母细胞中 Mrna 的定量和定位
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Use of Single Molecule Fluorescent In Situ Hybridization (SM-FISH) to Quantify and Localize mRNAs in Murine Oocytes
DOI:

08:18 min

April 24, 2019

,

Chapters

  • 00:04Title
  • 00:38Collection of Ovulated Oocytes from Female Mice
  • 02:06SM-FISH Staining of Oocytes
  • 04:21Image Processing
  • 06:21Results: mRNA Quantification Results using the Spot Finding and Tracking Program
  • 07:45Conclusion

Summary

Automatic Translation

为了可重复地计数单个卵母细胞中的 Mrna 数量, 对非粘附细胞的单分子 RNA 荧光原位杂交 (RNA-FISH) 进行了优化。卵母细胞被收集, 与转录特异性探针杂交, 并使用图像定量软件进行量化。

Related Videos

Read Article