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Lipidomics and Transcriptomics in Neurological Diseases
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Neuroscience
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JoVE Journal Neuroscience
Lipidomics and Transcriptomics in Neurological Diseases

Lipidomics and Transcriptomics in Neurological Diseases

DOI:
10.3791/59423-v

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09:58 min

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March 18, 2022

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, , , , ,
1Institute of Physiological Chemistry,University Medical Center of the Johannes Gutenberg University Mainz

Chapters

  • 00:04Introduction
  • 00:52Brain Punching
  • 02:20Co-extraction of eCBs and eiCs
  • 04:32Co-extraction of PLs, eCBs and Dual Extraction of RNA and Lipids
  • 07:51Results: Lipidomic and Transcription Profiling in KA-induced Epilepsy
  • 09:27Conclusion

Summary

Automatic Translation

Automatic Translation

This article presents a modular protocol for tissue lipidomics and transcriptomics, and plasma lipidomics in neurological disease mouse models targeting lipids underlying inflammation and neuronal activity, membrane lipids, downstream messengers, and mRNA-encoding enzymes/receptors underlying lipid function. Sampling, sample processing, extraction, and quantification procedures are outlined.

Tags

Keywords: LipidomicsTranscriptomicsNeurological DiseasesCryostatBrain SlicesBrain PunchesEndocannabinoidsEicosanoidsPhospholipidsLiquid-liquid ExtractionMass SpectrometryRNA ExtractionCentrifugationSample Preparation

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