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March 18, 2022
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This article presents a modular protocol for tissue lipidomics and transcriptomics, and plasma lipidomics in neurological disease mouse models targeting lipids underlying inflammation and neuronal activity, membrane lipids, downstream messengers, and mRNA-encoding enzymes/receptors underlying lipid function. Sampling, sample processing, extraction, and quantification procedures are outlined.
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Cite this Article
Post, J. M., Lerner, R., Schwitter, C., Lutz, B., Lomazzo, E., Bindila, L. Lipidomics and Transcriptomics in Neurological Diseases. J. Vis. Exp. (181), e59423, doi:10.3791/59423 (2022).
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