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Establishing Mouse Models for Zika Virus-induced Neurological Disorders Using Intracerebral Injection Strategies: Embryonic, Neonatal, and Adult
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Neuroscience
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JoVE Journal Neuroscience
Establishing Mouse Models for Zika Virus-induced Neurological Disorders Using Intracerebral Injection Strategies: Embryonic, Neonatal, and Adult

Establishing Mouse Models for Zika Virus-induced Neurological Disorders Using Intracerebral Injection Strategies: Embryonic, Neonatal, and Adult

DOI:
10.3791/56486-v

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09:39 min

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April 26, 2018

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, , , ,
1Biomedical and Health Sciences Institute,University of Georgia, 2Center for Craniofacial Molecular Biology,University of Southern California, 3Department of Infectious Diseases, College of Veterinary Medicine,University of Georgia

Chapters

  • 00:04Title
  • 01:07Intracerebral Zika Virus Inoculation in Embryos
  • 04:02Intracerebral Zika Virus Inoculation in Neonates
  • 06:06Intracerebral Zika Virus Inoculation in Adult Mice
  • 07:53Results: Representative Histology in ZIKV-inoculated Embryos, Neonates, and Adult Mice
  • 08:57Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a method for establishing a model of Zika virus-induced microcephaly in mouse. This protocol includes methods for embryonic, neonatal, and adult-stage intracerebral inoculation of the Zika virus.

Tags

Keywords: Zika VirusMouse ModelIntracerebral InjectionEmbryonicNeonatalAdultIn VivoBrain InfectionMicrocephalyNeuro-developmental DisordersFlavivirusDengue VirusSurgical ProcedureAnesthesiaEmbryo ExposureVirus Inoculation

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