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用于测试 Vivo 中巨噬菌体招募的异位化学表达模型
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Developmental Biology
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JoVE Journal
Developmental Biology
An Ectopic Chemokine Expression Model for Testing Macrophage Recruitment In Vivo
Please note that all translations are automatically generated.
Click here for the English version.
用于测试 Vivo 中巨噬菌体招募的异位化学表达模型
DOI:
10.3791/60161-v
•
10:02 min
•
September 25, 2019
•
Yunyun Jiang
,
Jiahao Chen
,
Jin Xu
1
Department of Developmental Biology, School of Basic Medical Sciences
,
Southern Medical University
,
2
Division of Cell, Developmental and Integrative Biology, School of Medicine
,
South China University of Technology
Chapters
00:04
Title
00:52
Fluorescent Whole Mount In Situ Hybridization (WISH) Combined with Immunostaining
05:33
Live Imaging
08:25
Results: Fluorescent WISH and Live Imaging
09:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
为了测试化学金对体内巨噬细胞招募的影响,使用整个原位杂交检测化学体异位表达,免疫染色用于标记巨噬细胞。实时成像用于实时观察巨噬菌体迁移。
Tags
Ectopic
Chemokine
Macrophage
Recruitment
In Vivo
Cell Chemotaxis
Zebrafish
TGFABP-10A
Interleukin-34
Transgenic
Mpeg1:GFP
Fixation
Dehydration
Rehydration
Proteinase K
Hybridization
Probe
Formamide
SSCT
Blocking Buffer
Anti-digoxigenin HRP Antibody
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