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利用生命病毒介导的人神经元转导研究头塔聚集的体外模型
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
Please note that all translations are automatically generated.
Click here for the English version.
利用生命病毒介导的人神经元转导研究头塔聚集的体外模型
DOI:
10.3791/59433-v
•
05:51 min
•
May 23, 2019
•
Brent Aulston
,
Qing Liu
,
Patrick Reilly
,
Shauna H. Yuan
1
Department of Neurosciences
,
University of California, San Diego
Chapters
00:04
Title
00:27
Preparation of Media and Reagents
01:41
Culturing Human Neural Stem Cells
03:12
Transduction and Maintenance of Neuronal Cultures
04:05
Live-cell Imaging
04:32
Results: Analysis of Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
05:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议详细介绍了用慢病毒结构编码为突变体人类 tau 转换人类神经元培养的过程。诱导的区域性显示 tau 聚合和相关的病理。
Tags
Tau Aggregation
In Vitro Model
Human Neurons
Lentiviral Transduction
Cell Culture
Drug Screening
Disease Mechanism
Basement Membrane Matrix
Neural Stem Cell Media
BFGF
DMEM-F12
Penicillin-streptomycin
Cell Thawing
Centrifugation
Cell Seeding
Confluency
Lentivirus Transduction
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