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En in vitro model for at studere Tau aggregering ved hjælp af Lentiviral-medieret transduktion af humane neuroner
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Neuroscience
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JoVE Journal Neuroscience
An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons

En in vitro model for at studere Tau aggregering ved hjælp af Lentiviral-medieret transduktion af humane neuroner

DOI:
10.3791/59433-v

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05:51 min

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May 23, 2019

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, , ,
1Department of Neurosciences,University of California, San Diego

Chapters

  • 00:04Title
  • 00:27Preparation of Media and Reagents
  • 01:41Culturing Human Neural Stem Cells
  • 03:12Transduction and Maintenance of Neuronal Cultures
  • 04:05Live-cell Imaging
  • 04:32Results: Analysis of Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
  • 05:22Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver en procedure, hvor menneskelige neuronal kulturer er transduceret med lentiviral konstruktioner kodning for mutant menneske Tau. Transduceret kulturer display Tau aggregater og tilknyttede patologier.

Tags

Tau AggregationIn Vitro ModelHuman NeuronsLentiviral TransductionCell CultureDrug ScreeningDisease MechanismBasement Membrane MatrixNeural Stem Cell MediaBFGFDMEM-F12Penicillin-streptomycinCell ThawingCentrifugationCell SeedingConfluencyLentivirus Transduction

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