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Un modelo in vitro para estudiar la agregación de Tau utilizando la transducción de neuronas humanas mediada por lentivirales
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
An In Vitro Model for Studying Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
DOI:

05:51 min

May 23, 2019

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Chapters

  • 00:04Title
  • 00:27Preparation of Media and Reagents
  • 01:41Culturing Human Neural Stem Cells
  • 03:12Transduction and Maintenance of Neuronal Cultures
  • 04:05Live-cell Imaging
  • 04:32Results: Analysis of Tau Aggregation Using Lentiviral-mediated Transduction of Human Neurons
  • 05:22Conclusion

Summary

Automatic Translation

Este protocolo detalla un procedimiento en el que las culturas neuronales humanas son transducidas con construcciones lentivirales codificando para tau humano mutante. Las culturas transducadas muestran agregados Tau y patologías asociadas.

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