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Biochemistry
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利用标准化定量西方印迹技术, 对跨组织和整个开发过程中的蛋白质水平进行可靠比较
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
Please note that all translations are automatically generated.
Click here for the English version.
利用标准化定量西方印迹技术, 对跨组织和整个开发过程中的蛋白质水平进行可靠比较
DOI:
10.3791/59438-v
•
08:13 min
•
April 09, 2019
•
Yu-Ting Huang
2
,
Dinja van der Hoorn*
1,2
,
Leire M. Ledahawsky*
1,2
,
Anna A. L. Motyl*
1,2
,
Crispin Y. Jordan
,
Thomas H. Gillingwater
2
,
Ewout J. N. Groen
2
1
Centre for Discovery Brain Sciences
,
University of Edinburgh
,
2
Euan MacDonald Centre for Motor Neurone Disease Research
,
University of Edinburgh
Chapters
00:04
Title
00:34
Protein Extraction
01:15
Quantification and Normalization of Protein Concentration
02:22
Protein Sample Gel Electrophoresis and Protein Transfer
04:03
Total Protein Staining
05:26
Image Acquisition and Western Blot Analysis
06:39
Results
07:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方法描述了一种可靠且可重复的方法, 用于使用标准化的定量西方印迹法比较不同组织和不同发育时间点的蛋白质水平。
Tags
Protein Levels
Quantitative Western Blotting
Standardized Protocol
Developmental Analysis
Fluorescent Total Protein Stain
Internal Loading Control
Protein Extraction
Protein Quantification
Electrophoresis
Gel Loading
Between-membrane Normalization
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