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Biochemistry
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通过对Rho激酶活性的暂时抑制,增强人类诱导多能干细胞衍生心肌细胞的诱导
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Biochemistry
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JoVE Journal
Biochemistry
Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity
Please note that all translations are automatically generated.
Click here for the English version.
通过对Rho激酶活性的暂时抑制,增强人类诱导多能干细胞衍生心肌细胞的诱导
DOI:
10.3791/59452-v
•
08:00 min
•
July 10, 2019
•
Meng Zhao
,
Yawen Tang
,
Patrick J. Ernst
2
,
Asher Kahn-Krell
,
Chengming Fan
3
,
Danielle Pretorius
,
Hanxi Zhu
,
Xi Lou
,
Lufang Zhou
,
Jianyi Zhang
,
Wuqiang Zhu
1
Department of Biomedical Engineering, School of Medicine, School of Engineering
,
University of Alabama at Birmingham
,
2
Division of Cardiovascular Diseases, School of Medicine, School of Engineering
,
University of Alabama at Birmingham
,
3
Department of Cardiac Surgery
,
The Second Xiangya Hospital of Central South University
Chapters
00:04
Title
01:13
hiPSC-CMs Purification, Small Molecule Pre-treatment, Myocardial Infarction, and Cell Transplantation
03:42
Calcium Transient and Contractility Recording
05:21
Results: Increased Engraftment of Transplanted hiPSC-CM Preconditioned with the ROCK Inhibitor Y-27632 in a Murine MI Model
07:11
Conclusion
Summary
Automatic Translation
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Automatic Translation
在该协议中,我们演示并阐述了如何使用人类诱导多能干细胞进行心肌细胞分化和纯化,并进一步说明如何利用Rho相关蛋白激酶抑制剂提高移植效率小鼠心肌梗死模型的预处理。
Tags
HiPSC-derived Cardiomyocytes
Rho Kinase Activity
Cell Engraftment
Cell Adhesion
Cell Survival
Cardiac Infarction
Heart Failure
Y-27632
Verapamil
Cell Transplantation
Cell Dissociation
Cell Culture
Cardiac Function
Vascularization
Apoptosis
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