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Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
JoVE Journal
Cancer Research
Author Produced
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JoVE Journal Cancer Research
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
DOI:

11:44 min

March 30, 2019

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Chapters

  • 00:00Title
  • 01:08Transfection
  • 03:08RNA Extraction
  • 04:53cDNA Synthesis and qPCR
  • 06:28Cell Cycle Analysis
  • 08:13Antibody Protein Microarray
  • 09:34Representative Results
  • 11:09Conclusion

Summary

Automatic Translation

miRNA therapeutics have significant potential in regulating cancer progression. Demonstrated here are analytical approaches used for identification of the activity of a combinatorial miRNA treatment in halting cell cycle and angiogenesis.

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