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Journal / Neuroscience / In Vivo Direkte Reprogrammierung von Resident…
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Neuroscience

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JoVE Journal Neuroscience
In Vivo Direkte Reprogrammierung von Resident Glial Cells in Interneurons durch Intracerebral Injection of Viral Vectors
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In Vivo Direkte Reprogrammierung von Resident Glial Cells in Interneurons durch Intracerebral Injection of Viral Vectors

Article DOI: 10.3791/59465-v • 12:26 min • June 17th, 2019
June 17th, 2019 •
1, 1, 1
1Department of Experimental Medical Sciences and Lund Stem Cell Center, BMC, Lund University

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Dieses Protokoll zielt darauf ab, direkt umprogrammierte Interneuronen in vivo zu erzeugen, indem ein AAV-basiertes Virussystem im Gehirn und ein FLEX synapsingesteuerter GFP-Reporter verwendet werden, der die Zellidentifikation und weitere Analyse in vivo ermöglicht.

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Neurowissenschaften Ausgabe 148 direkte Umwandlung Ganzzell-Patchklemme FLEX-Vektorsystem Cre-induzierbares System Slice-Aufnahmen Parvalbumin Transdifferenzierung neuronale Umwandlung
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