JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
亚细胞分辨率的各向同性光片显微镜及细胞自动动力学分析
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution
Please note that all translations are automatically generated.
Click here for the English version.
亚细胞分辨率的各向同性光片显微镜及细胞自动动力学分析
DOI:
10.3791/59533-v
•
08:16 min
•
June 06, 2019
•
Leighton H. Duncan
5
,
Mark W. Moyle
5
,
Lin Shao
5
,
Titas Sengupta
5
,
Richard Ikegami
5
,
Abhishek Kumar
5
,
Min Guo
5
,
Ryan Christensen
5
,
Anthony Santella
5
,
Zhirong Bao
5
,
Hari Shroff
5
,
William Mohler
5
,
Daniel A. Colón-Ramos
5,6
1
Department of Neuroscience and Department of Cell Biology
,
Yale University School of Medicine
,
2
Developmental Biology Program
,
Sloan Kettering Institute
,
3
Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling
,
University of Connecticut Health Center
,
4
Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering
,
National Institutes of Health
,
5
WormGUIDES.org
,
6
Instituto de Neurobiología, Recinto de Ciencias Médicas
,
Universidad de Puerto Rico
Chapters
00:04
Title
00:34
C. elegans
Embryo Sample Mounting Preparation
01:48
Dual-View Inverted Selective Plane Illumination Microscopy (diSPIM) Embryonic Imaging
03:12
Isotropic High-Resolution Volumetric Data: Cell Lineage Analysis
06:06
Results: Representative Cellular Identification and Single Cell Characterization of Neurite Outgrowth Dynamics
07:43
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里, 我们提出了一个组合方法, 使用高分辨率显微镜, 计算工具, 和单细胞标记在活的
线虫
胚胎, 以了解单个细胞动力学在神经发育过程中。
Tags
Isotropic Light-sheet Microscopy
Caenorhabditis Elegans
Embryogenesis
Cell Lineage Analysis
Subcellular Resolution
DiSPIM
Live Imaging
Neuron Morphology
M9 Buffer
Methylcellulose
Micromanager
Autofocus
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
生殖系化学毒性的利用线虫综合评估<em>秀丽隐杆线虫</em
采用轻质板材荧光显微镜到图像斑马鱼眼睛发育
细胞谱系分析和基因功能研究使用双点MARCM
光片为主的生活的荧光显微镜或固定和染色<em>赤拟谷盗</em>胚胎
高分辨率显微镜(HREM) - 用于处理和可视化有机材料的简单和可靠的协议
斑马鱼视网膜蛋白定位的相关超分辨和电镜观察
固定化的
线虫线虫
分析神经元内细胞的转运
评估溶酶体碱化在活的
秀丽线虫
中的肠道
线虫
中耳炎群链球菌引起氧化应激的研究
全山荧光在小鼠胚胎发生过程中心脏室发育的分析
Read Article