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Developmental Biology
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等方性光シート顕微鏡と自動細胞系譜分析による、細胞内分解能を Caenorhabditis elegans 胚発生
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution
Please note that all translations are automatically generated.
Click here for the English version.
等方性光シート顕微鏡と自動細胞系譜分析による、細胞内分解能を Caenorhabditis elegans 胚発生
DOI:
10.3791/59533-v
•
08:16 min
•
June 06, 2019
•
Leighton H. Duncan
5
,
Mark W. Moyle
5
,
Lin Shao
5
,
Titas Sengupta
5
,
Richard Ikegami
5
,
Abhishek Kumar
5
,
Min Guo
5
,
Ryan Christensen
5
,
Anthony Santella
5
,
Zhirong Bao
5
,
Hari Shroff
5
,
William Mohler
5
,
Daniel A. Colón-Ramos
5,6
1
Department of Neuroscience and Department of Cell Biology
,
Yale University School of Medicine
,
2
Developmental Biology Program
,
Sloan Kettering Institute
,
3
Department of Genetics and Genome Sciences and Center for Cell Analysis and Modeling
,
University of Connecticut Health Center
,
4
Section on High Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering
,
National Institutes of Health
,
5
WormGUIDES.org
,
6
Instituto de Neurobiología, Recinto de Ciencias Médicas
,
Universidad de Puerto Rico
Chapters
00:04
Title
00:34
C. elegans
Embryo Sample Mounting Preparation
01:48
Dual-View Inverted Selective Plane Illumination Microscopy (diSPIM) Embryonic Imaging
03:12
Isotropic High-Resolution Volumetric Data: Cell Lineage Analysis
06:06
Results: Representative Cellular Identification and Single Cell Characterization of Neurite Outgrowth Dynamics
07:43
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここでは、
elegans
胚における高分解能顕微鏡、計算ツール、および単一細胞標識法を用いて、neurodevelopment 中の単一細胞動態を理解するための組合せアプローチを提示する。
Tags
Isotropic Light-sheet Microscopy
Caenorhabditis Elegans
Embryogenesis
Cell Lineage Analysis
Subcellular Resolution
DiSPIM
Live Imaging
Neuron Morphology
M9 Buffer
Methylcellulose
Micromanager
Autofocus
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