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Isotropic lys-ark mikroskopi og automatisert Cell Lineage analyser til katalog Caenorhabditis elegans embryogenesis med Subcellulære oppløsning
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Isotropic Light-Sheet Microscopy and Automated Cell Lineage Analyses to Catalogue Caenorhabditis elegans Embryogenesis with Subcellular Resolution
DOI:

08:16 min

June 06, 2019

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Chapters

  • 00:04Title
  • 00:34C. elegans Embryo Sample Mounting Preparation
  • 01:48Dual-View Inverted Selective Plane Illumination Microscopy (diSPIM) Embryonic Imaging
  • 03:12Isotropic High-Resolution Volumetric Data: Cell Lineage Analysis
  • 06:06Results: Representative Cellular Identification and Single Cell Characterization of Neurite Outgrowth Dynamics
  • 07:43Conclusion

Summary

Automatic Translation

Her presenterer vi en Kombinatorisk tilnærming ved hjelp av høyoppløselig mikroskopi, beregningsorientert verktøy, og enkelt celle merkingsteknikker i å leve C. elegans embryo å forstå enkelt celle dynamikk under neurodevelopment.

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