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Neuroscience
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マウス脳スライスにおける長距離入力の光遺伝学的刺激のための生体内脳内立体注射
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices
Please note that all translations are automatically generated.
Click here for the English version.
マウス脳スライスにおける長距離入力の光遺伝学的刺激のための生体内脳内立体注射
DOI:
10.3791/59534-v
•
09:07 min
•
September 20, 2019
•
Louis Richevaux
2
,
Louise Schenberg
2
,
Mathieu Beraneck
2
,
Desdemona Fricker
2
1
CNRS (Integrative Neuroscience and Cognition Center, UMR 8002)
,
2
Université Paris Descartes, Sorbonne Paris Cité
Chapters
00:04
Title
00:42
Stereotaxic Surgery
04:01
Preparation of Brain Slices
05:28
Whole-Cell Patch-Clamp Recording
07:21
Results: Presubicular Layer III Neuron: Intrinsic Properties, Responses to Light Stimulation, and Post-hoc Revelation of Cell Morphology
08:19
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは、ex vivo脳スライスにおける光遺伝学的刺激を用いて、遠方の脳領域からの長距離入力の細胞型特異的機能的接続性を同定する一連の方法を説明する。
Tags
In Vivo
Intracerebral
Stereotaxic
Injections
Optogenetic
Stimulation
Long-range Inputs
Mouse Brain Slices
Functional Connectivity
Photostimulation
Axons
Stereotaxic Targeting
Lidocaine Hydrochloride
Craniotomy
Viral Solution
Hamilton Syringe
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