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在Vivo脑内立体注射中，用于小鼠脑切片中远距离输入的光遗传学刺激
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Neuroscience
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JoVE Journal Neuroscience
In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices
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在Vivo脑内立体注射中，用于小鼠脑切片中远距离输入的光遗传学刺激

DOI:
10.3791/59534-v

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09:07 min

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September 20, 2019

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, , ,
1CNRS (Integrative Neuroscience and Cognition Center, UMR 8002), 2Université Paris Descartes, Sorbonne Paris Cité

Chapters

  • 00:04Title
  • 00:42Stereotaxic Surgery
  • 04:01Preparation of Brain Slices
  • 05:28Whole-Cell Patch-Clamp Recording
  • 07:21Results: Presubicular Layer III Neuron: Intrinsic Properties, Responses to Light Stimulation, and Post-hoc Revelation of Cell Morphology
  • 08:19Conclusion

Summary

Automatic Translation

Automatic Translation

该协议描述了一组方法，利用外体脑切片中的光遗传学刺激，确定来自遥远大脑区域的远程输入的细胞类型特定功能连接。

Tags

In VivoIntracerebralStereotaxicInjectionsOptogeneticStimulationLong-range InputsMouse Brain SlicesFunctional ConnectivityPhotostimulationAxonsStereotaxic TargetingLidocaine HydrochlorideCraniotomyViral SolutionHamilton Syringe

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