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Flow Cytometrisk analyse af mitokondrie reaktive oxygen arter i murine hæmatopoietiske stamceller og Progenitorcellerne og MLL-AF9 drevet leukæmi
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Chapters
- 00:05Title
- 01:46Mitochondrial ROS Fluorogenic Dye Staining
- 04:41Lineage Antibody Staining
- 06:04Flow Cytometry Acquisition and Analysis
- 07:24Results: Flow Cytometry Gating Strategies and Mitochondrial ROS Levels
- 08:54Conclusion
Vi beskriver en metode til brug af multiparametrflow cytometri til at detektere mitokondrie reaktive oxygenarter (ROS) i murine sund hæmatopoietisk stamcelle-og progenitorceller (Hspc'er) og leukæmiceller fra en musemodel af akut myeloid leukæmi (AML) drevet af MLL-AF9.
