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Studera Organelledynamik i B-celler under Immunsynapsebildningen
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Studying Organelle Dynamics in B Cells During Immune Synapse Formation
DOI:

15:39 min

June 01, 2019

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Chapters

  • 00:00Title
  • 01:16B Cell Activation with Antigen-coated Beads
  • 02:48B Cell Activation on Antigen-coated Coverslips
  • 03:51Immunofluoresence and Image Analysis
  • 04:30Analysis of the Distribution of Organelles in B Cells Activated with Ag-coated Beads
  • 07:42Analysis of Cell Spreading and Distribution of Organelles in B Cells Activated on Ag-coated Coverslips
  • 09:29Isolation of Centrosome-enriched Fractions from Resting and Activated B Cells
  • 11:23Results
  • 15:07Conclusion

Summary

Automatic Translation

Häri beskriver vi två metoder för att karakterisera cellpolariseringshändelser i B-lymfocyter under bildandet av en IS. Den första, innebär kvantifiering av organell rekrytering och cytoskelettet rearrangemang vid Synaptic membranet. Den andra är en biokemisk metod, att karakterisera förändringar i sammansättningen av centrosome, som genomgår polarisering till immun synapsen.

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