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JoVE Journal
Immunology and Infection

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Detecting Cortex Fragments During Bacterial Spore Germination

Detecting Cortex Fragments During Bacterial Spore Germination

Article DOI: 10.3791/54146 08:35 min June 25th, 2016
June 25th, 2016



Herein, we describe a colorimetric assay to detect the presence of reducing sugars during bacterial spore germination.



Detecting Cortex Fragments Bacterial Spore Germination Quantifying Abundance Reducing Sugars Protocol Detect Presence Advantage Technique Laboratories Reagents Equipment Clostridium Difficile Spores Heating Block Incubation Ice Germination Solution Tris Sodium Chloride Glycine Taurocholic Acid Deionized Water Microcentrifuge Tube Blank Sample OD600
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