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JoVE Journal
Biochemistry

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用于研究拉帕霉素/mTOR相互作用的半定量药物亲和反应目标稳定性 (DARTS) 测定
 
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用于研究拉帕霉素/mTOR相互作用的半定量药物亲和反应目标稳定性 (DARTS) 测定

Article DOI: 10.3791/59656-v
August 27th, 2019

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Summary August 27th, 2019

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在这项研究中,我们通过监测蛋白质稳定性的变化和估计蛋白质-配体相互作用的亲和力,增强了DARTS实验的数据分析能力。相互作用可以绘制成两条曲线:蛋白质解解曲线和剂量依赖曲线。我们使用 mTOR-拉帕霉素相互作用作为示例案例。

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