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Rask og effektiv uttrykk for flere proteiner i avian embryo bruke mRNA electroporation
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1Department of Biological Sciences,University of Southern California, Los Angeles, 2Department of Radiology and Developmental Neuroscience Program, Saban Research Institute,Children’s Hospital Los Angeles, 3Keck School of Medicine, Department of Radiology,University of Southern California, Los Angeles
Chapters
- 00:04Title
- 00:55Living Quail Embryo Preparation
- 02:50Electroporation-encoded Fluorescent Protein (FP) Imaging
- 05:44Fluorescence Recovery After Photobleaching (FRAP)
- 07:35Results: Representative Electroporation-encoded FP Expression in Avian Embryos
- 09:21Conclusion
Vi rapporterer Messenger RNA (mRNA)-electroporation som en metode som tillater rask og effektiv uttrykk for flere proteiner i modell systemet vaktel embryo. Denne metoden kan brukes å fluorescensmerkete avmerke celler og fortegnelse deres inne vivo bevegelser av tid-lapse mikroskopi kort etter electroporation.
