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使用mRNA电穿孔快速有效地表达Avian胚胎中的多种蛋白质
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation
DOI:

09:40 min

June 07, 2019

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Chapters

  • 00:04Title
  • 00:55Living Quail Embryo Preparation
  • 02:50Electroporation-encoded Fluorescent Protein (FP) Imaging
  • 05:44Fluorescence Recovery After Photobleaching (FRAP)
  • 07:35Results: Representative Electroporation-encoded FP Expression in Avian Embryos
  • 09:21Conclusion

Summary

Automatic Translation

我们报告信使RNA(mRNA)电穿孔作为一种方法,允许快速有效地表达多个蛋白质在龟胚胎模型系统。该方法可用于荧光标记细胞,并在电穿孔后不久通过延时显微镜记录细胞体内运动。

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