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Developmental Biology
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使用mRNA电穿孔快速有效地表达Avian胚胎中的多种蛋白质
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Developmental Biology
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JoVE Journal
Developmental Biology
Fast and Efficient Expression of Multiple Proteins in Avian Embryos Using mRNA Electroporation
Please note that all translations are automatically generated.
Click here for the English version.
使用mRNA电穿孔快速有效地表达Avian胚胎中的多种蛋白质
DOI:
10.3791/59664-v
•
09:40 min
•
June 07, 2019
•
Martin Tran
,
Mohit Dave
3
,
Rusty Lansford
2,3
1
Department of Biological Sciences
,
University of Southern California, Los Angeles
,
2
Department of Radiology and Developmental Neuroscience Program, Saban Research Institute
,
Children’s Hospital Los Angeles
,
3
Keck School of Medicine, Department of Radiology
,
University of Southern California, Los Angeles
Chapters
00:04
Title
00:55
Living Quail Embryo Preparation
02:50
Electroporation-encoded Fluorescent Protein (FP) Imaging
05:44
Fluorescence Recovery After Photobleaching (FRAP)
07:35
Results: Representative Electroporation-encoded FP Expression in Avian Embryos
09:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们报告信使RNA(mRNA)电穿孔作为一种方法,允许快速有效地表达多个蛋白质在龟胚胎模型系统。该方法可用于荧光标记细胞,并在电穿孔后不久通过延时显微镜记录细胞体内运动。
Tags
MRNA Electroporation
Avian Embryo
Protein Expression
Fluorescent Proteins
Transient Transfection
Developmental Stage
Electroporation Chamber
Epiblast
Vitelline Membrane
Fluorescent Imaging
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