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Hurtig og effektiv ekspression af flere proteiner i aviær embryoner ved hjælp af mRNA-elektroporation
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1Department of Biological Sciences,University of Southern California, Los Angeles, 2Department of Radiology and Developmental Neuroscience Program, Saban Research Institute,Children’s Hospital Los Angeles, 3Keck School of Medicine, Department of Radiology,University of Southern California, Los Angeles
Chapters
- 00:04Title
- 00:55Living Quail Embryo Preparation
- 02:50Electroporation-encoded Fluorescent Protein (FP) Imaging
- 05:44Fluorescence Recovery After Photobleaching (FRAP)
- 07:35Results: Representative Electroporation-encoded FP Expression in Avian Embryos
- 09:21Conclusion
Vi rapporterer Messenger RNA (mRNA) elektroporation som en metode, der tillader hurtig og effektiv ekspression af flere proteiner i vagtler embryo modelsystemet. Denne metode kan anvendes til fluorescently label celler og registrere deres in vivo bevægelser ved time-lapse mikroskopi kort efter elektroporation.
