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用螺旋粘度计对人血小板和细胞表面受体进行均匀剪切检测
JoVE Journal
Biology
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JoVE Journal Biology
A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry
DOI:

04:32 min

June 05, 2019

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Chapters

  • 00:04Title
  • 00:34Platelet Isolation
  • 01:15Ligand and Uniform Shear Treatment and Surface Marker Detection
  • 02:49Results: Representative Platelet Activation Marker Expression
  • 04:01Conclusion

Summary

Automatic Translation

我们描述了一种用邻面色谱法将均匀剪切应用于血小板表面受体的溶液法。这种方法也可以更广泛地应用剪切其他细胞类型和细胞片段, 不需要针对特定的配体受体对。

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