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Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates
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Immunology and Infection
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JoVE Journal Immunology and Infection
Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates

DOI:
10.3791/59759-v

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07:43 min

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July 22, 2019

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,
1Department of Marine Sciences,University of North Carolina, Chapel Hill

Chapters

  • 00:04Title
  • 00:28Preparation of Each Bacterial Strain for Incubation
  • 01:21Coincubation of Strains
  • 02:54Serial Dilution for Starting Inoculum
  • 04:37Taking T Final Measurements
  • 05:36Results: Quantifying Competitive Interactions Between the Strains
  • 07:26Conclusion

Summary

Automatic Translation

Automatic Translation

Bacteria encode diverse mechanisms for engaging in interbacterial competition. Here, we present a culture-based protocol for characterizing competitive interactions between bacterial isolates and how they impact the spatial structure of a mixed population.

Tags

Vibrio FischeriCoincubation AssayCompetitive InteractionsAntibiotic ResistanceFluorescent ProteinsCell SuspensionOptical DensityNormalizationDifferential TaggingColony Forming UnitsFluorescence Microscopy

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