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Journal
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Immunology and Infection
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用于量化
Vibrio Fischeri
分离物之间竞争交互的联合孵化测定
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Coincubation Assay for Quantifying Competitive Interactions between
Vibrio fischeri
Isolates
Please note that all translations are automatically generated.
Click here for the English version.
用于量化
Vibrio Fischeri
分离物之间竞争交互的联合孵化测定
DOI:
10.3791/59759-v
•
07:43 min
•
July 22, 2019
•
Lauren Speare
,
Alecia N. Septer
1
Department of Marine Sciences
,
University of North Carolina, Chapel Hill
Chapters
00:04
Title
00:28
Preparation of Each Bacterial Strain for Incubation
01:21
Coincubation of Strains
02:54
Serial Dilution for Starting Inoculum
04:37
Taking T Final Measurements
05:36
Results: Quantifying Competitive Interactions Between the Strains
07:26
Conclusion
Summary
Automatic Translation
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Automatic Translation
细菌编码各种机制,参与细菌间竞争。在这里,我们提出了一个基于培养的协议,用于描述细菌分离物之间的竞争相互作用,以及它们如何影响混合种群的空间结构。
Tags
Vibrio Fischeri
Coincubation Assay
Competitive Interactions
Antibiotic Resistance
Fluorescent Proteins
Cell Suspension
Optical Density
Normalization
Differential Tagging
Colony Forming Units
Fluorescence Microscopy
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