Journal
/
Biology
/
Rensning af blodplader fra muse blod
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Purification of Platelets from Mouse Blood

Rensning af blodplader fra muse blod

DOI:
10.3791/59803-v

•

05:41 min

•

May 07, 2019

•

,
1Division of Experimental Hematology and Cancer Biology,Cincinnati Children’s Hospital Medical Center, 2University of Cincinnati College of Medicine

Chapters

  • 00:04Title
  • 00:37Platelet Purification
  • 01:31Platelet Activation
  • 02:51Flow Cytometric Platelet Analysis
  • 03:30Results: Representative Platelet Purification Evaluation
  • 05:03Conclusion

Summary

Automatic Translation

Automatic Translation

Her, mus blod blev indsamlet i nærværelse af en anti-koagulans. Trombocytterne blev renset ved Iohexol gradient medium ved hjælp af lav hastighed centrifugering. Trombocytterne blev aktiveret med thrombin for at undersøge, om de var levedygtige. Kvaliteten af de rensede blodplader blev analyseret ved flow cytometri og mikroskopi.

Tags

Platelet PurificationMouse BloodIohexol GradientLow-speed CentrifugationPlatelet-rich LayerPlatelet ActivationGPRP PeptideThrombinFlow CytometryForward ScatterSide Scatter

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Isolation of CD4+ T cells from Mouse Lymph Nodes Using Miltenyi MACS Purification

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid

Purification of Mitochondria from Yeast Cells

Isolation and Culture of Pulmonary Endothelial Cells from Neonatal Mice

Purification of Progenitors from Skeletal Muscle

Flow Cytometry Purification of Mouse Meiotic Cells

Isolering og oprensning af kinesin fra Drosophila Embryoner

Generering af mus afledt fra induceret pluripotente stamceller

Oprensning og microRNA profilering af Exosomer basis af blod og Kultur Medier

Celleoverflademarkør Mediated Oprensning af iPS Cell Mellemprodukter fra en Programmerbar musemodel

Read Article