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在Vivo免疫荧光定位中用于评估癌症研究中的治疗和诊断抗体生物分布
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Cancer Research
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JoVE Journal Cancer Research
In Vivo Immunofluorescence Localization for Assessment of Therapeutic and Diagnostic Antibody Biodistribution in Cancer Research
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在Vivo免疫荧光定位中用于评估癌症研究中的治疗和诊断抗体生物分布

DOI:
10.3791/59810-v

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08:53 min

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September 16, 2019

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,
1Apoptosis, Cancer and Development Laboratory – Equipe labellisée ‘La Ligue’, LabEx DEVweCAN, Centre de Cancérologie de Lyon,INSERM U1052-CNRS UMR5286, Centre Léon Bérard, 2Department of Radiology/Molecular Imaging Program at Stanford, School of Medicine,Stanford University

Chapters

  • 00:04Title
  • 00:50Intravenous Injection of Specific and Nonspecific Antibody Agents
  • 02:27Collection and Preparation of Target Tumor Tissues
  • 03:53Ex Vivo Staining Protocol
  • 06:07Results: IVIL Shows Varying Biodistribution Patterns of Anti-B7-H3 and Anti-Netrin-1 Antibodies in Murine Mammary Tumors In Vivo Compared to Respective Control Antibodies
  • 07:50Conclusion

Summary

Automatic Translation

Automatic Translation

体内免疫荧光定位（IVIL）方法可用于利用体内肿瘤靶向和体外免疫染色的组合，在活体中检测抗体和抗体结合物在体内的生物体中的抗体和抗体结合方法。

Tags

In Vivo Immunofluorescence LocalizationIVILTherapeutic AntibodyDiagnostic AntibodyBiodistributionCancer ResearchTumor GrowthTail Vein Inoculation

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