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使用生物素标签研究蛋白质-核酸相互作用的体外生化分析
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In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
Please note that all translations are automatically generated.
Click here for the English version.
使用生物素标签研究蛋白质-核酸相互作用的体外生化分析
DOI:
10.3791/59830-v
•
08:14 min
•
July 17, 2019
•
Lina Yu*
1
,
Wenxiu He*
1
,
Jie Xie*
1
,
Rui Guo*
1
,
Juan Ni
,
Xia Zhang
,
Quishi Xu
,
Caifeng Wang
,
Qiuling Yue
,
Fangfang Li
,
Mengcheng Luo
,
Bo Sun
,
Lan Ye
,
Ke Zheng
1
State Key Laboratory of Reproductive Medicine
,
Nanjing Medical University
,
2
The Affiliated Hospital of Hangzhou Normal University
,
3
School of Life Science and Technology
,
ShanghaiTech University
,
4
Department of Tissue and Embryology, School of Basic Medical Sciences, Hubei Provincial Key Laboratory of Developmentally Originated Disease
,
Wuhan University
Chapters
00:04
Title
00:40
Protein Preparation
03:25
Nucleic Acid Preparation and In Vitro Biochemical Assay
05:22
Chemiluminescence Detection
06:18
Results: Interaction of MEIOB and MOV10 Proteins with Nucleic Acids
07:49
Conclusion
Summary
Automatic Translation
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Automatic Translation
这里介绍的是使用生物素标签的体外生化测定方案,可广泛用于研究蛋白质-核酸相互作用。
Tags
Biotin Labels
Protein-nucleic Acid Interactions
In Vitro Biochemical Assays
MEIOB
MOV10
Affinity Purification
GST-tagged Proteins
FLAG-tagged Proteins
Centrifugation
Cell Lysis
Magnetic Beads
Elution
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