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Genetics
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ビオチン標識を用いてタンパク質核酸相互作用を研究するインビトロ生化学アッセイ
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JoVE Journal
Genetics
In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
Please note that all translations are automatically generated.
Click here for the English version.
ビオチン標識を用いてタンパク質核酸相互作用を研究するインビトロ生化学アッセイ
DOI:
10.3791/59830-v
•
08:14 min
•
July 17, 2019
•
Lina Yu*
1
,
Wenxiu He*
1
,
Jie Xie*
1
,
Rui Guo*
1
,
Juan Ni
,
Xia Zhang
,
Quishi Xu
,
Caifeng Wang
,
Qiuling Yue
,
Fangfang Li
,
Mengcheng Luo
,
Bo Sun
,
Lan Ye
,
Ke Zheng
1
State Key Laboratory of Reproductive Medicine
,
Nanjing Medical University
,
2
The Affiliated Hospital of Hangzhou Normal University
,
3
School of Life Science and Technology
,
ShanghaiTech University
,
4
Department of Tissue and Embryology, School of Basic Medical Sciences, Hubei Provincial Key Laboratory of Developmentally Originated Disease
,
Wuhan University
Chapters
00:04
Title
00:40
Protein Preparation
03:25
Nucleic Acid Preparation and In Vitro Biochemical Assay
05:22
Chemiluminescence Detection
06:18
Results: Interaction of MEIOB and MOV10 Proteins with Nucleic Acids
07:49
Conclusion
Summary
Automatic Translation
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Automatic Translation
ここに示すビオチン標識を用いてインビトロ生化学アッセイを用い、タンパク質-核酸相互作用の研究に広く適用可能なプロトコルを示す。
Tags
Biotin Labels
Protein-nucleic Acid Interactions
In Vitro Biochemical Assays
MEIOB
MOV10
Affinity Purification
GST-tagged Proteins
FLAG-tagged Proteins
Centrifugation
Cell Lysis
Magnetic Beads
Elution
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