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来自成人大脑的短期自由浮动切片培养
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Short-Term Free-Floating Slice Cultures from the Adult Human Brain
DOI:

09:14 min

November 05, 2019

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Chapters

  • 00:04Title
  • 01:14Setting Up the Slicing Apparatus
  • 02:14Sample Collection
  • 02:45Slicing
  • 04:14Culture
  • 05:18Morphological Evaluation
  • 06:39Results: Analysis of the Short-Term Free-Floating Slice Cultures
  • 08:12Conclusion

Summary

Automatic Translation

提出了一种从成人大脑中制备自由浮动切片培养物的协议。该协议是使用膜插入物广泛使用的切片培养方法的变体。它简单,经济高效,建议进行短期检测,旨在解开与年龄相关的脑疾病背后的神经退化机制。

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