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Kortsigtede fritflydende udsnit kulturer fra den voksne menneskelige hjerne
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1Department of Biochemistry and Immunology, Ribeirão Preto Medical School,University of São Paulo, 2Department of Physiology, Ribeirão Preto Medical School,University of São Paulo, 3Department of Pathology and Forensic Medicine, Ribeirão Preto Medical School,University of São Paulo, 4Department of Anatomy, Institute of Biosciences,São Paulo State University, 5Clinical Hospital at the Ribeirão Preto Medical School,University of São Paulo
Chapters
- 00:04Title
- 01:14Setting Up the Slicing Apparatus
- 02:14Sample Collection
- 02:45Slicing
- 04:14Culture
- 05:18Morphological Evaluation
- 06:39Results: Analysis of the Short-Term Free-Floating Slice Cultures
- 08:12Conclusion
En protokol til at forberede fritflydende skive kulturer fra voksne menneskelige hjerne er præsenteret. Protokollen er en variation af den udbredte skive kultur metode ved hjælp af membran skær. Det er simpelt, omkostningseffektivt, og anbefales til at kørekort sigtede assays sigter mod at opklare mekanismer af neurodegeneration bag aldersrelaterede hjernesygdomme.
