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JoVE Journal
Immunology and Infection

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Intravital Imaging af Intraepitelial lymfocytter i murine tyndtarmen
 
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Intravital Imaging af Intraepitelial lymfocytter i murine tyndtarmen

Article DOI: 10.3791/59853
June 24th, 2019

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Summary June 24th, 2019

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Vi beskriver en metode til at visualisere GFP-mærket γδ IELs ved hjælp af intravital billeddannelse af murine tyndtarmen ved inverteret spinning disk confokal mikroskopi. Denne teknik gør det muligt at spore levende celler i slimhinden i op til 4 timer og kan bruges til at undersøge en række af intestinal immun-epitheliale interaktioner.

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