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洗涤剂辅助重组重组果蝇阿拉他素到脂质混合测定脂质体
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Detergent-assisted Reconstitution of Recombinant Drosophila Atlastin into Liposomes for Lipid-mixing Assays
DOI:

08:43 min

July 03, 2019

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Chapters

  • 00:04Title
  • 00:56Liposome Production by Extrusion Method
  • 02:45Reconstitution by Detergent Assisted Incorporation into Preformed Liposomes
  • 05:10Lipid Mixing Assays
  • 06:16Results: Analysis of Liposomes Reconstituted from Recombinant Drosophila Atlastin
  • 07:58Conclusion

Summary

Automatic Translation

生物膜融合由专用融合蛋白催化。通过脂质混合测定,可以测量蛋白质的富源性。我们提出了一种纯化重组果蝇蛋白的方法,这是一种蛋白质,可以调节ER的同质融合,将其重组为预制脂质体,并测试融合能力。

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