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Journal
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Biochemistry
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洗涤剂辅助重组重组
果蝇阿
拉他素到脂质混合测定脂质体
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Detergent-assisted Reconstitution of Recombinant
Drosophila
Atlastin into Liposomes for Lipid-mixing Assays
Please note that all translations are automatically generated.
Click here for the English version.
洗涤剂辅助重组重组
果蝇阿
拉他素到脂质混合测定脂质体
DOI:
10.3791/59867-v
•
08:43 min
•
July 03, 2019
•
Miguel A. Betancourt-Solis
,
James A. McNew
1
Department of BioSciences
,
Rice University
Chapters
00:04
Title
00:56
Liposome Production by Extrusion Method
02:45
Reconstitution by Detergent Assisted Incorporation into Preformed Liposomes
05:10
Lipid Mixing Assays
06:16
Results: Analysis of Liposomes Reconstituted from Recombinant Drosophila Atlastin
07:58
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
生物膜融合由专用融合蛋白催化。通过脂质混合测定,可以测量蛋白质的富源性。我们提出了一种纯化重组果蝇蛋白的方法,这是一种蛋白质,可以调节ER的同质融合,将其重组为预制脂质体,并测试融合能力。
Tags
Detergent-assisted Reconstitution
Recombinant Drosophila Atlastin
Liposomes
Lipid-mixing Assays
FRET-based Lipid-mixing
Proteoliposomes
Lipid Film
Freeze-thaw
Extrusion
Lipid Concentration
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