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Presynapse dannelse analyse ved hjælp af Presynapse Organizer perler og "neuron Ball" kultur
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Neuroscience
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JoVE Journal Neuroscience
Presynapse Formation Assay Using Presynapse Organizer Beads and “Neuron Ball” Culture

Presynapse dannelse analyse ved hjælp af Presynapse Organizer perler og "neuron Ball" kultur

DOI:
10.3791/59893-v

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10:17 min

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August 02, 2019

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, ,
1Functional Structure Biology Laboratory, Department of Medical Life Science,Yokohama City University Graduate School of Medical Life Science

Chapters

  • 00:04Title
  • 00:55Preparation of Neuron Balls as Hanging Drop Culture
  • 03:24Placing Neuron Balls on PLL-Coated Glass Coverslips and Culture Maintenance
  • 04:29Applying LRRTM2 Beads on Neuron Ball Culture With or Without Cell Bodies
  • 06:07Fluorescence Microscopy
  • 07:19Results: Analysis of Accumulation of Presynaptic Proteins in LRRTM2-Induced Presynapses of Axons of Neuron Ball Culture
  • 09:15Conclusion

Summary

Automatic Translation

Automatic Translation

Presynapse dannelse er en dynamisk proces, herunder ophobning af synaptiske proteiner i ordentlig orden. I denne metode, presynapse dannelse udløses af perler konjugeret med en presynapse organisator protein på axonal plader af "neuron Ball" kultur, således at ophobning af synaptiske proteiner er let at blive analyseret under presynapse dannelse.

Tags

Presynapse FormationPresynapse Organizer BeadsNeuron Ball CultureSynapse FormationPresynaptic ProteinsCortical Neuron CultureEmbryonic Mouse BrainTrypsinizationTriturationHanging Drop Culture

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