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Journal
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Biochemistry
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极快速和特殊的代谢标签的RNA在Vivo与4-Thiouracil (Ers4tU)
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)
Please note that all translations are automatically generated.
Click here for the English version.
极快速和特殊的代谢标签的RNA在Vivo与4-Thiouracil (Ers4tU)
DOI:
10.3791/59952-v
•
00:11 min
•
August 22, 2019
•
J. David Barrass
,
Jean D. Beggs
1
Wellcome Centre for Cell Biology, School of Biological Sciences
,
University of Edinburgh
Chapters
00:04
Title
00:38
Growth and Thio-labeling
02:55
Preparation of Total RNA
04:38
Biotinylation
05:58
Purification of the Newly Synthesized RNA
09:29
Results: In Vivo Labelling of RNA with 4-Thiouracil
10:44
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
使用硫化尿素来敏感和专门地从酵母
糖母体
中纯化新转录的RNA。
Tags
4-Thiouracil
RNA Labeling
In Vivo
Pulse-chase Experiments
Protein Depletion
Thiolation
Yeast
S. Pombe
Metabolic Labeling
RNA Extraction
AE Buffer
Phenol Extraction
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