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Immunology and Infection

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在自身免疫糖尿病模型中高效生成抗原特异性原小鼠细胞毒性T细胞细胞细胞，用于功能测试

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Immunology and Infection

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JoVE Journal Immunology and Infection

High-Efficiency Generation of Antigen-Specific Primary Mouse Cytotoxic T Cells for Functional Testing in an Autoimmune Diabetes Model

Please note that all translations are automatically generated. Click here for the English version.

在自身免疫糖尿病模型中高效生成抗原特异性原小鼠细胞毒性T细胞细胞细胞，用于功能测试

DOI:

10.3791/59985-v

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11:31 min

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August 16, 2019

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Howard W. Davidson, Joseph Ray Cepeda, Nitin S. Sekhar, Junying Han, Ling Gao, Tomasz Sosinowski, Li Zhang

¹Barbara Davis Center for Childhood Diabetes,University of Colorado Denver, ²Department of Medicine, Endocrinology, Diabetes & Metabolism,Baylor College of Medicine, ³Scientific Center,Shandong Provincial Hospital affiliated to Shandong University

Chapters

00:04Title
01:00Transfection of Viral Producer Cells
03:34Primary CD8 T Cell Isolation and Activation
07:10Transduction of Activated CD8 T Cells
09:29Results: Analysis of Transduced CD8 CAR-T Cells
10:42Conclusion

Summary

Automatic Translation

本文介绍了一种用于生成抗原特异性CD8 T细胞及其在体外扩张的协议，目的是产生大量功能性T细胞，用于体外和体内。

Tags

Keywords: Antigen-specific Primary Mouse Cytotoxic T Cells Autoimmune Diabetes High-efficiency Transduction CAR T Cells Cancer Immune Cells Phoenix Cells Transfection Reduced Serum Medium Packaging Plasmid Virus IL-2

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