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Journal
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Cancer Research
/
腫瘍抗原特異的T細胞の生成にヒト誘導多能性幹細胞を用いた
JoVE Journal
Cancer Research
This content is Free Access.
JoVE Journal
Cancer Research
Using Human Induced Pluripotent Stem Cells for the Generation of Tumor Antigen-specific T Cells
Please note that all translations are automatically generated.
Click here for the English version.
腫瘍抗原特異的T細胞の生成にヒト誘導多能性幹細胞を用いた
DOI:
10.3791/59997-v
•
10:57 min
•
October 24, 2019
•
Meghan L. Good*
1,2
,
Raul Vizcardo*
1,2
,
Takuya Maeda
2
,
Naritaka Tamaoki
2
,
Parisa Malekzadeh
,
Hiroshi Kawamoto
,
Nicholas P. Restifo
2
1
Surgery Branch
,
National Cancer Institute, NIH
,
2
Center for Cell-Based Therapy
,
National Cancer Institute, NIH
,
3
Laboratory of Immunology, Institute for Frontier Life and Medical Sciences
,
Kyoto University
Chapters
00:04
Title
00:58
Passaging Human iPSCs on Mouse Embryonic Fibroblasts
02:30
Preparation of OP9/DLL1 Cells for Co-culture with hiPSCs
03:51
In Vitro Differentiation of hiPSCs into CD8αβ
+
08:38
Results: hiPSC-derived Hematopoietic Progenitor Cells
09:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本稿では、OP9/DLL1共培養系を用いて機能性腫瘍抗原特異的人工多能性幹細胞
由来CD8αβ+単陽性T細胞を生成する方法について説明する。
Tags
Human Induced Pluripotent Stem Cells
Tumor Antigen-specific T Cells
OP9-Delta-1 Co-culture
T Cell Generation
Regenerative Medicine
Cell-based Therapy
NK Cells
Cell Differentiation
Cell Passaging
Mouse Embryonic Fibroblasts
OP9 Media
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