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Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment

Article DOI: 10.3791/60420-v • 11:11 min • January 24th, 2020
January 24th, 2020 •
1,2,3, 4, 4,5, 4,6,7, 1,2, 1,2, 4, 4
1Department of Urology, Wan Fang Hospital, 2Department of Urology, School of Medicine, College of Medicine, Taipei Medical University, 3Graduate Institute of Clinical Medicine, College of Medicine, Taipei Medical University, 4Translational Cell Therapy Center, Department of Medical Research, China Medical University Hospital, 5Graduate Institute of Biomedical Sciences, China Medical University, 6Graduate Institute of Immunology, China Medical University, 7Department of Neurosurgery, Neuropsychiatric Center, China Medical University Hospital

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Summary
Automatic Translation

Here, we present a protocol to perform the isolation and expansion of peripheral blood mononuclear cells-derived cytokine-induced CD3+CD56+ killer cells and illustrate their cytotoxicity effect against hematological and solid cancer cells by using an in vitro diagnosis flow cytometry system.

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Isolation Expansion Cytotoxic Cytokine-induced Killer T Cells Cancer Treatment PBMC-derived Cytotoxicity Standard Operating Procedure Technicians Clinicians Scientific Research Clinical Evaluation Preparation Wash Centrifuge Resuspend Cell Numbers Viability Test Trypan Blue Exclusion Assay Aliquot Density Label Treat Antibodies Incubate
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