Journal
/
Cancer Research
/
Using the Chicken Chorioallantoic Membrane In Vivo Model to Study Gynecological and Urological Cancers
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Using the Chicken Chorioallantoic Membrane In Vivo Model to Study Gynecological and Urological Cancers

Using the Chicken Chorioallantoic Membrane In Vivo Model to Study Gynecological and Urological Cancers

DOI:
10.3791/60651-v

•

08:13 min

•

January 28, 2020

•

, , , ,
1Molecular and Medical Pharmacology,University of California Los Angeles

Chapters

  • 00:04Introduction
  • 00:49Egg Opening
  • 04:00Cancer Cell Suspension Preparation
  • 04:42Non-Stick Ring Implantation
  • 05:44Implantation Without a Non-Stick Ring
  • 06:40Results: Representative Establishment of Cancer Cell Engraftment Using the CAM Model
  • 07:40Conclusion

Summary

Automatic Translation

Automatic Translation

We present the chicken chorioallantoic membrane model as an alternative, transplantable, in vivo model for the engraftment of gynecological and urological cancer cell lines and patient-derived tumors.

Tags

Keywords: Chorioallantoic MembraneCAM ModelIn VivoTumorTumorigenicityTreatment EfficacyCellular CrosstalkTherapeutic ApproachesCell LinesPatient Tumor SpecimensAir CellVasculatureVascular WindowEgg ShellInner MembraneIncubator

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/60651?article_type=v

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion
check_url/60651?article_type=v

How to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases
check_url/60651?article_type=v

The Drosophila Imaginal Disc Tumor Model: Visualization and Quantification of Gene Expression and Tumor Invasiveness Using Genetic Mosaics
check_url/60651?article_type=v

Live Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers
check_url/60651?article_type=v

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples
check_url/60651?article_type=v

Detection of Mitochondria Membrane Potential to Study CLIC4 Knockdown-induced HN4 Cell Apoptosis In Vitro
check_url/60651?article_type=v

In Vivo EPR Assessment of pH, pO2, Redox Status, and Concentrations of Phosphate and Glutathione in the Tumor Microenvironment
check_url/60651?article_type=v

Acellular and Cellular Lung Model to Study Tumor Metastasis
check_url/60651?article_type=v

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
check_url/60651?article_type=v

Comparing Metastatic Clear Cell Renal Cell Carcinoma Model Established in Mouse Kidney and on Chicken Chorioallantoic Membrane

Read Article