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Journal
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Developmental Biology
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神経クレスト細胞の形成と移動の研究のためのマウスからの原発性頭蓋神経クレスト細胞の解剖、培養および分析
JoVE Journal
Developmental Biology
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JoVE Journal
Developmental Biology
Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
Please note that all translations are automatically generated.
Click here for the English version.
神経クレスト細胞の形成と移動の研究のためのマウスからの原発性頭蓋神経クレスト細胞の解剖、培養および分析
DOI:
10.3791/60051-v
•
09:33 min
•
October 03, 2019
•
Sandra Guadalupe Gonzalez Malagon*
1,2
,
Lisa Dobson*
1,3
,
Anna M Lopez Muñoz
,
Marcus Dawson
,
William Barrell
3
,
Petros Marangos
4
,
Matthias Krause
,
Karen J Liu
1
Centre for Craniofacial and Regenerative Biology
,
King’s College London
,
2
Institute of Molecular Biology and Biotechnology, FORTH, Department of Biomedical Research
,
University of Ioannina
,
3
Randall Centre of Cell & Molecular Biophysics
,
King’s College London
,
4
Department of Biological Applications and Technology
,
University of Ioannina
Chapters
00:04
Title
01:05
Early Somite Stage Embryo Dissection (Day 1)
03:12
Live Cell Imaging of Murine Cranial Neural Crest Cells (Day 2)
04:36
Neural Crest Single Cell Tracking
05:22
Quantification of Neural Crest Cell Migratory Capacity
06:04
Quantification of Neural Crest Cell Area and Circularity
07:00
Results: Representative Cranial Neural Crest Cell Migration and Cell Shape Dynamics Analyses
08:43
Conclusion
Summary
Automatic Translation
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Automatic Translation
このプロトコルは、主に細胞移動の研究のために、マウスモデルからの頭蓋神経堤細胞の解剖および培養について説明する。使用されるライブイメージング技術と速度と細胞形状の変化の解析について述べた。
Tags
Primary Cranial Neural Crest Cells
Neural Crest Cell Delamination
Neural Crest Cell Migration
Neural Plate
Embryonic Dissection
Neural Crest Development
Mouse Embryo
Cell Culture
Developmental Biology
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